HPLC determination of synthetic dyes in animal tissues

1 HPLC determination of synthetic dyes in animal tissues 

RP-HPLC-vis measurements were performed in an ODS column (250 X 2 mm i.d. particle size 5 jum). A 10 X 2 mm column filled with lead(IV) oxide was employed for the post-column oxidation of the analytes. The method of post-column oxidation allowed the simultaneous detection of each dye and metabolite in the visible range. Dyes were eluted with a two-phase step gradient programme mobile phase 1 (0.05 M aqueous ammonium 

Recoveries for HPLC-vis varied between 69.1-71.3 per cent (MG) with RSD values 4.4-7.0 per cent. The same values for CV were 87.0-95.5 per cent and 8.1-16.5 per cent, respectively. The validity parameters of LMG (91.2-97.3 per cent, RSD 2.3-14.3 per cent) and LCV (96.4-114.5 per cent RSD 3.0-23.6 per cent) were similar. The LOD values were in general lower than 1 /xg/kg. 

It was concluded from the measurements that the extraction method is rapid and simple and allows the reproducible and sensitive determination of the dyes by both HPLC-vis andHPLC-MS techniques [101], 

Another RP-HPLC method has also been developed and applied for the determination of MG, LMG, gentian violet (GV) and leuco gentian violet (LCV) in catfish and trout tissues. The chemical structures of MG and LMG were previously presented. The chemical structure of VG and LVG are shown in Fig. 3.21. Dyes were extracted mixing 20.0 g of 

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