HPLC-CVAAS

Figure 6.1 Bar-graph of MeHg in CRM 580. The results correspond to six replicate determinations as performed by different laboratories using various methods. MEANS indicates the mean of laboratory means with 95% confidence interval. Abbreviations-. CVAAS, cold vapour atomic absorption spectrometry CVAFS, cold vapour atomic fluorescence spectrometry ECD, electron capture detection GC, gas chromatography HPLC, high-performance liquid chromatography ICPMS, inductively coupled plasma mass spectrometry MIP, microwave induced plasma atomic emission spectrometry QFAAS, quartz furnace atomic absorption spectrometry SFE, supercritical fluid extraction.

Falter, R. and H.F. Scholer. 1995. Determination of mercury species in natural waters at pictogram level with on-line RP C18 preconcentration and HPLC-UV-PCO-CVAAS. Fresenius J. Anal. Chem. 353 ... 

Total Hg determined by CVAAS after digestion with HNO, for 4 h at 80°C, and reduction with SnCU- For MeHg, 1000 mg was pre-treated by addition of HCI and toluene, back-extraction with thiosulphate, addition of acetate buffer, complexation and on-line oxidation (after HPLC), and reduction with SnCI, separation was by high performance liquid chromatography (HPLC), followed by CVAFS detection (Lab.OS)... 

The results for Pig 1 state, The presence of CVAOA was confirmed by HPLC/IC which found 20 ppm CVAOA and 40 ppm CVAA in the sample. The results for Pig 2 state, The presence of DM was also confirmed by the large amount of arsenic found in the sample (>5000 ppm). Current arsenic removal guidelines require the removal of arsenic contaminated soil above 20 ppm, irrespective of depth. 

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