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HPLC Column-operating Guides

The following operating guides are recommended for maintaining reversed-phase columns. Consult the vendor s column instructions for precautions and column regeneration guides. [Pg.259]

Store the columns in acetonitrile or methanol or in a mixture of water and organic solvents. Cap them with closed fittings to prevent the columns from drying out. [Pg.259]

Do not exceed the pH range of the column (typically pH of 2.5 to 8 for silica-based columns). Silica dissolves at pH greater than 8 and bonded groups can hydrolyze at pH less than 2. Note that many modern silica-based columns may have more durable bonding chemistries for extended pH usable range of 2-10 or even 1-12.10  [Pg.259]

Do not exceed the temperature limits of most columns, usually 60-80 C, without consulting the instructions from the column vendor. [Pg.260]

Always flush the column with a strong solvent (methanol) before use to eliminate any highly retained analyte. [Pg.260]


The following operating guides are recommended for connecting HPLC columns ... [Pg.260]


See other pages where HPLC Column-operating Guides is mentioned: [Pg.255]    [Pg.259]    [Pg.255]    [Pg.259]    [Pg.226]    [Pg.255]   
See also in sourсe #XX -- [ Pg.259 ]




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