HPLC and ultracentrifugation

The results obtained by the two methods were compared and a correlation for the corresponding lipoprotein classes in the two methods was very good for all lipoprotein classes in both groups, except for VHOL. The individual data for each lipoprotein class as measured by the HPLC method (X) and the ultracentrifugal method (Y) are shown in Fig. 18. The correlation coefficients of the least square analysis are as follows 0.91 - 0.94 for chylomicrons+VLDL, 0.97 -0.98 for LDL, 0.96 for HDL, 0.83 - 0.87 for HDLj and 0.59 for VHDL in a group of normolipidemia (n=15) 0.98 for chylomicrons+VLDL, 0.99 for LDL, 0.98 for HDL, 0.88 - 0.97 for HOL and 0.44 for VHDL in a group of hyperlipidemia (n=12). 

However, this good correlation for the lipoprotein analysis between the two methods dose not exist when the size of lipoproteins no longer corresponds to the density as proved by a comparison of the results for a liver disease group (n=20) by Okazaki et al. (31), and by a study of lecithin cholesterol acyltrans-ferase deficiency by Kodama et al. (32). 

---