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How to run a thin-layer chromatogram

Prepare the TLC development tank using a clean, dry beaker, as shown in Fig. 32.11, and allow it to equilibriate for 10 minutes. [Pg.218]

Prepare the plastic backed TLC plate by drawing a fine line (the base line) in pencil about 1 cm above the bottom. Take care not to scrape off any of the stationary phase. Put three pencil dots on the base line - one in the centre and the others equidistant on either side, but no closer than 3 mm to the edges of the plate. [Pg.218]

3 Dissolve the mixture (1-3 mg) in two or three drops of a suitable volatile solvent (dichloromethane or ether are the most common), in a small sample tube. [Pg.218]

Is capillary action will draw the solution into the pipette. [Pg.218]

Touch the tip of the micropipette onto one of the pencil spots on the base line. Capillary action will draw the solution from the migropipette onto the plate as a spot. Do not allow the spot be mdre than 2 mm in diameter. Put the micropipette back into the sample tube containing the mixture. [Pg.218]


See other pages where How to run a thin-layer chromatogram is mentioned: [Pg.218]    [Pg.218]    [Pg.371]   


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