How Does UmuC V Insert dATP

Other G A mispairings are also possible. In prindple, anti-dATP can pair with. vyrt-guanirie in adduct-dG, which requires the pyrene moiety to be in the major groove. Anti-dATP can also pair with anti-guanine in adduct-dG in an elongated mispair. Thus, there are scenarios other than the one involving our syn-dATP anti-adduct-dG hypothesis. 

Although the dislocation/templated mechanism is attractive for Dpo4, considerable evidence both in vitro and in vivo suggests that neither Pol IV nor V follow a dislocation/templated mechanism with +BP (discussed extensively in [116], as well as in [79, 93, 113]). 

This analysis suggests a reason for caution when applying conclusions from Dpo4 to other Y-family DNA polymerases, especially those purely in the IV/k or V/q class. Perhaps Dpo4 evolved its hybrid roof/chimney structure to bypass a unique set of lesions encountered by a thermophilic bacterium. Alternatively, perhaps the structure of Dpo4 at physiologically relevant elevated temperatures is 

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