Horseradish peroxidase voltammetry

R. Huang and N.F. Hu, Direct voltammetry and electrochemical catalysis with horseradish peroxidase in polyacrylamide hydrogel films. Biophys. Chem 104,199-208 (2003). 

Note HRP Horseradish peroxidase TMB Tetramethylbenzidine ACV Alternating-current voltammetry amole le-18 moles DPV DifTerential pulse voltammetry E Detection limit for unconjugated enzyme E Detection limit for conjugated (Ab/Ag) enzyme FIA-EC Flow-injection analysis with electrochemical detection CC Classy carbon LC-EC Liquid chromatography with electrochemical detection P Detection limit for final enzymatic product P Detection limit for standards of P RDE Rotating disk electrode SPE Screen-printed electrode SQV Square-wave voltammetry zmole le-21 moles. 

A significant challenge is to use amperometry or voltammetry to detect redox-inactive molecules. Enzyme-modified electrodes can play a significant role in this area. For example, a combination of horseradish peroxidase and L-glutamate oxidase [20] immobilized within a redox hydrogel can be used to monitor L-glutamate secretion from hippocampal neurons [21]. 

Yu et al. have covalently linked myoglobin and horseradish peroxidase to an aligned SWCNT electrode through the amide linkage. Quasi-reversible Ee +/Ee + voltammetry was observed for the iron heme enzymes, myoglobin, and horseradish peroxidase. The results demonstrated that the trees in the nanotube forest are potent current collectors that conduct electrons from the external circuit to the redox sites of the enzymes. 

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