HK97 capsid

Fold HK97, capsid protein has two unique domains, both with an antiparallel sheet and helices, an extended... 

Fig. 2. Thermolysin rapidly cleaves the highly interlinked capsid from Hong Kong 97 virus at 65°C. Cleavage reaction was performed for 2 h in 100 mmol/1 Tris-HCl, 10 mmol/1 CaCl2. Trypsin cleavage showed no significant fragmentation within this time. Inset-, an image of HK97...

Fig. 8. HK97 assembly and maturation. (A) Negatively stained electron micrograph of a mature dsDNA-filled capsid, with noncontractile tail and accessory proteins. (B) Steps in capsid assembly and maturation (see text in vitro conditions are in boldface, and in vivo conditions or components that differ from the in vitro conditions are in italic) (Conway et al, 1995). (C) Chemistry of the cross-linking reaction.

We suggest that the expansion transformation involves a subunit rotation mechanism, as visualized at the level of a quasi-atomic model for phage HK97 (Conway et al, 2001). Specifically, rotation of gplO subunits about axes in the plane of the capsid shell and extending radially outward from the local symmetry axis may bring densities that were formerly on the inner surface into the plane of the shell. To accommodate these densities, the centers of adjacent capsomers are pushed further apart. Concomitantly, the gp9 scaffold protein subunits are released. Both of these effects have the effect of making the mature capsid thinner walled and smoother surfaced than its precursor. 

Hetpesviridae family as, 244—245 HK97 head and, 175 P22 tailspin protein and, 176, 177 papillomavirns and, 172 polyomavimses and, 171—72, 245 PRDl capsid protein and, 175 T4fibritin/baseplate proteins and, 176—78 29 motor protein and, 175—76 Donble-stranded RNA virnses, 240—246 endogenons transcription/exit pathways of 231-232... 

Chapman and Liljas, Fig. 16. The capsid protein of phage HK97 (Wikoff et al.,... 

Conway JF, Duda RL, Cheng N, Htaidrix RW, Steven AC (1995) Proteolytic and conformational control of virus capsid maturatimi the bacteriophage HK97 system. J Mol Biol 253 86-99... 

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