Hippocampal slice temperature

Mitani A., Kadoya F., and Kataoka K. (1991) Temperature dependence of hypoxia-induced calcium accumulation in gerbil hippocampal slices. Brain Res. 562, 159-163. 

Reid, K. H., Schurr, A., and West, C. A. (1987) Effects of duration of hypoxia, temperature and aCSF potassium concentration on probability of recovery of CAl synaptic function in the in vitro rat hippocampal slice, in Brain Slices Fundamentals, Applications and Implications (Schurr, A., Teyler, T. J, and Tseng, M. T., eds.), Karger, Basel, pp. 143-146. 

Although it has always been rumored that the response of the hippocampal slice is neither uniform nor linear between temperatures of 32 and 37°C, only recently have data been published to support this suspicion (unpublished data of Skrede, Teyler, and Westgaard, reported by Teyler, 1980). As shown in Fig. 6 the most dramatic change in excitability occurs around 37°C. Clearly, even minute changes in temperature around this point will cause changes in the tissue response, and thus... 

Fig. 6. The relationship between hippocampal excitability and ambient temperature. Guinea pig slices were prepared in the usual way and incubated in standard media and perforant path stimulation held constant at a level where a 5 mV population spike was recorded from the cell body layer. In (A) are shown records recorded at five different temperatures, and (B) shows the time course of the experiment. Note at time 0 the temperature was 28°C and no activity could be elicited from the hippocampus. As the temperature was raised activity was increased until it reached a maximum of near 40 C. The temperature was then slowly lowered and the entire cycle repeated once more. At temperatures above 40°C the response declined precipitiously. In (C) the amplitude of the cumulative population spike is plotted as a function of temperature. From Skrede, Teyler and Westgaard (unpublished, quoted from Teyler, 1980.)...

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