High-throughput screening negatives

The 3T3 NRU test may easily be performed under GLP conditions in contract research organizations (CROs) and a high-throughput screening (HTS) method was recently reported in the literature. The HTS method produced no false positives, although some false negatives were observed, suggesting that the standard 3T3 NRU test protocol remains necessary for the final selection of nonphototoxic compounds [42]. 

Dalvit, C., et al., NMR-based quality control approach for the identification of false positives and false negatives in high throughput screening. Curr Drug Discov Technol, 2006, 3, 115-124. 

Zebrafish 50-200 (lateral line) AG, CP, LD, AM + HC regenerate + High-throughput screens - False-negative ototoxins identified - Only one HC type - Immature characteristics of HCs... 

In contrast to false positives, false negatives are relatively frequent in protein crystallography, in particular when dealing with low to very low affinity hits from high-throughput screening (HTS) or FBS, for reasons which will... 

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