High-Throughput Experimental Methods

The constantly increasing amount of data coming from high throughput experimental methods, from genome sequences, from functional- and structural genomics has given a rise to a need for computer-assisted methods to elucidate sequence-structure-function relationships. 

For additional discussion of laboratory techniques, see Liquid-Liquid Equilibrium Experimental Methods as well as High-Throughput Experimental Methods under Solvent-Screening Methods. ... 

In a recent work, formation of block multipolymers from radicalized VDC copolymer particulates has been demonstrated (Caneba et al., 2008). Using the two-stage stirred-tank reactor system (Fig. 4.2.1), recipes and conditions were generated using high-throughput experimentation methods. From cloudpoint experiments (Table 1.1.3), it has already been established that the solvent for the VDC polymerization via FRRPP process is azeotropic MEK/i-butanol. 

Combinatorial chemistry, 7 380-434 8 400—401 13 283-284. See also High-throughput experimentation applications, 7 381-383 commercial environment, 7 387-389 methodology, 7 383-387 microwaves in, 16 548-552 nomenclature, 7 380 polymers, 7 405—413 Combinatorial libraries, 12 515-517 Combinatorial methods, 7 380 Combinatorial optimization approach, in computer-aided molecular design, 26 1037... 

The last two decades have seen enormous developments in catalyst discovery and optimization tools, notably in the area of high-throughput experimentation (HTE) and process optimization (5). However, the basic concept used for exploring the catalyst space in homogeneous catalysis has not changed Once an active catalyst complex is discovered, small modifications are made on the structure to try and screen the activity of neighboring complexes, covering the space much like an ink drop spreads on a sheet of paper. This is not a bad method, but can we do better with the new tools that are available today ... 

In the last decade methods of combinatorial catalysis and high throughput experimentation has obtained great interest [1-4]. In the field of heterogeneous catalysis most of the efforts are devoted to the investigation of gas phase reactions, where several hundreds catalysts can simultaneously be tested [5,6]. Contrary to that, in high-pressure liquid phase catalytic reactions in a single reactor module only 8-16 parallel experiments can be performed. There are reports to use up to six modules as a parallel setup [7]. 

Although the list of analysis techniques available for Stage I screening is quite notable, there is still great demand for new methods to enhance high-throughput experimentation. Especially high is the need for truly parallel techniques, preferably optical, to enable very short analysis times. 

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