High-Speed Two-Dimensional Capillary Electrophoresis

Our initial experiments required about 8 h to complete the two-dimensional separation. This long period results in drift due to temperature changes and buffer evaporation. We have modified the instrument to generate faster separations (Kraly et al., 2006). Shorter and narrower inner-diameter capillaries are now employed, which allow operation at 1000 V/cm, and dramatically improves the separation time. We employ a 10 s second-dimension separation period, and the separation is now complete in 40 min. 

FIGURE 15.6 Gel image of the data in Fig. 15.4, where the density of the plot is proportional to the logarithm of the fluorescence intensity observed in each cycle. The data are plotted as a gray scale for low intensity components and a blue-green-yellow-red scale for the most intense components. 

FIGURE 15.7 Landscape image of the data of Fig. 15.4. The height of each point is proportional to the intensity observed at that time. 

FIGURE 15.8 Expanded view. The data of Fig. 15.7 have been expanded by a factor of 30 to highlight components of intermediate intensity. 

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