High-performance tangential-flow filtration

Zydney, A.L. and van Reis, R., High performance tangential flow filtration, in Membrane Separations in Biotechnology, 2nd edn., W.K. Wang, (Ed.), Marcel Dekker, New York, 2001, pp. 277-298. 

R. van Reis, J.M. Brake, J. Charkoudian, D.B. Bums, A.L. Zydney, High performance tangential flow filtration using charged membranes, J. Membr. Sci. 159(1999) 133-142. 

HPTFF high-performance tangential-flow filtration... 

Van Reis et al. (1999) found that high separation factors for solute species with size differences less than an order of magnitude were obtainable using high-performance tangential-flow filtration. Their results indicate that maintaining a constant transmembrane pressure drop across the entire length of the membrane leads to a much finer fractionation of proteins. 

Comparing Figures 20.5a and 20.5b it can be seen that the sharpness of the virus particle fractionation is better with the 0.1-pm pore size membrane. Since membranes have a pore size distribution, development of a practical membrane-based fractionation step will depend on careful selection of an appropriate membrane pore size and pore size distribution. Further it is likely that operating methods such as high-performance tangential-flow filtration may improve the sharpness of the fractionation. 

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