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High-performance liquid separation efficiency

High performance liquid chromatography (HPLC) is an excellent technique for sample preseparation prior to GC injection since the separation efficiency is high, analysis time is short, and method development is easy. An LC-GC system could be fully automated and the selectivity characteristics of both the mobile and stationary... [Pg.304]

The spectrum of new analytical techniques includes superior separation techniques and sophisticated detection methods. Most of the novel instruments are hyphenated, where the separation and detection elements are combined, allowing efficient use of materials sometimes available only in minute quantities. The hyphenated techniques also significantly increase the information content of the analysis. Recent developments in separation sciences are directed towards micro-analytical techniques, including capillary gas chromatography, microbore high performance liquid chromatography, and capillary electrophoresis. [Pg.386]

Ishizuka, N., Kobayashi, H., Minakuchi, H., Nakanishi, K., Hirao, K., Hosoya, K., Ikegami, T., Tanaka, N. (2002). Monolithic silica columns for high-efficiency separations by high-performance liquid chromatography. J. Chromatogr. A 960, 85-96. [Pg.173]

Nimura, N., Itoh, H., Kinoshita, T., Nagae, N., Nomura, M. (1991). Fast protein separation by reversed-phase high-performance liquid-chromatography on octadecylsilyl-bonded non-porous silica-gel — effect of particle-size of column packing on column efficiency. J. Chromatogr. 585(2), 207-211. [Pg.240]

Particulate sorbents are available almost exclusively in the shape of micrometersized beads. These beads are packed in columns and represent currently the most common stationary phases for high-performance liquid chromatography (HPLC). Despite their immense popularity, slow diffusional mass transfer of macromolecular solutes into the stagnant pool of the mobile phase present in the pores of the separation medium and the large void volume between the packed particles are considered to be major problems in the HPLC of macromolecules, frequently impairing their rapid and efficient separation [1]. [Pg.89]

Arki, A. et al.. High-performance liquid chromatographic separation of stereoisomers of P-amino acids and a comparison of separation efficiencies on CHIROBIOTIC T and TAG columns, Chromatographia, 60, S43, 2004. [Pg.169]

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