High-performance liquid chromatography guard columns

Capparella, M. Foster, W., Ill Larrousse, M. Phillips, D.J. Pomfret, A. Tuvim, Y. Characteristics and applications of a new high-performance liquid chromatography guard column. J.Chromatogr.A, 1995, 691, 141-150... 

High-performance liquid chromatography (HPLC) system with refractive index detector and HPLC column (e.g., Aminex HPX-87H, Bio-Rad) fitted with suitable guard column... 

A typical high-performance liquid chromatography (HPLC) system consists of two pumps, a mixer, an injector, a guard colunm, a separation column, a detector, an electrospray nozzle, and a mass spectrometer. The two pumps and the mixer allow establishment of desired solvent gradients. The injector is used to inject a small amount of sample into the column. In all separation techniques, definition of a small volume of injected sample is cmcial in preventing adverse broadening of peaks and a consequent loss in compmient resolution. The injected compounds are separated in the separation colunm and then detected via a simple detector or a mass spectrometer. All of the parts of the HPLC system have been miniaturized, and several research groups have been able to demonstrate on-chip HPLC separations. 

A typical high-performance liquid chromatography (HPLC) system consists of two pumps, a mixer, an injector, a guard column, a separation column, a detector, an electrospray nozzle and a mass spectrometer. The two pumps and the mixer allow establishment of desired solvent gradients. The injector is used to inject a small... 

As in the case for gas chromatography, the column and detector are the heart of the high performance liquid chromatograph. There are many types of columns used for liquid chromatography. These are classified as guard, derivatizing, capillary, fast, and preparatory columns. 

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