High performance liquid chromatography flow programming

Fig. 4 schematically shows a design of the centrifugal partition chromatographic system (see Fig. 2). The separation disk has a series of partition chambers connected by narrow ducts. The disk is rotated up to 2000 rpm. The flow-through system is made by a pair of rotary seals, which can maintain leak-free elution up to 60 bar ( 800 psi), although the seals should be kept clean. The system is computerized and the operation is programed as in an high-performance liquid chromatography (HPLC) system.

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