High-performance liquid chromatography column design

Unfortunately, most column and sorbent manufacturers do not develop column packing materials mainly for SEC work, but for the bigger high-performance liquid chromatography (HPEC) market. However, there are many important differences to consider when designing packings for different modes of chroma-... 

High-performance liquid chromatography (HPLC) is the method for detection, identification and also quantification of flavonoids, phenolic acids and their derivatives. With this method, the sample is applied and eluted through a chromatographic column under specific conditions designed for optimum separation and resolution so that each compound or group of compound passes through the column with a... 

A high-performance liquid chromatography (HPLC) procedure was developed to analyze the exudate since the constituents absorbed strongly in the UV. Reverse phase chromatography (y Bondapak C.g column with a 90Z acetonitrile-10% 0.1 N acetic acid-water mobile phase) resulted in the separation of two peaks designated A and B. ... 

If the columns are well packed with small particles and liquid mobile phases are used, a successful technique, designated as high-performance liquid chromatography (HPLC), is frequently applied. 

The starting point for this chapter is that of the definition of HPLC (high-performance liquid chromatography) adopted by the author namely, that this is a method of separation of the components of a solution effected by the chromatographic process involving a column of solid stationary phase material and a liquid mobile phase in which all of the various components of the system have been designed so as to optimize the separation. In this context, optimization means first and foremost the complete (quantitative) separation of the components but it can also include such separations that are rapid and which have been automated. The key word in this treatment of instrumentation for HPLC is that of design. 

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