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High inositol phosphates analysis

Rounds, M.A. and Nielsen, S. S., Anion-exchange high-performance liquid chromatography with post-column detection for the analysis of phytic acid and other inositol phosphates, /. Chromatogr. A, 653, 148, 1993. [Pg.284]

There are two main procedures for measuring PI 3-kinase activity which measure lipid kinase activity in intact cells or broken cell lysates respectively, and both rely on detecting the transfer of the y- phosphate of ATP to the D-3 position of the inositol head group of phosphoinositide lipids. The first method relies on metabolic labeling of intact cellular pools of ATP with [32P]Pi followed by lipid extraction (3,4) and separation of the phosphorylated lipids by high-performance liquid chromatography (HPLC) analysis (5). The advantages of this procedure are ... [Pg.164]

See other pages where High inositol phosphates analysis is mentioned: [Pg.50]    [Pg.188]    [Pg.334]    [Pg.767]    [Pg.428]    [Pg.86]    [Pg.295]    [Pg.244]    [Pg.257]    [Pg.317]    [Pg.768]    [Pg.1484]    [Pg.280]   
See also in sourсe #XX -- [ Pg.2 , Pg.5 , Pg.13 , Pg.41 , Pg.45 ]



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