High energy fragmentation

MS/MS Duty Cycle Typical MS/MS analysis is a serial process, relying on the selection of precursors (peptides) in MS mode, followed by high-energy fragmentation in MS/MS. This process is termed data dependent acquisition (DDA). The duty cycle for the completion of MS and MS/MS cycles (the time necessary for MS/MS spectrum acquisition) is of primary importance. When the separation performance is viewed from the mass spectrometry perspective, the peak capacity can be characterized by the number of MS/MS scans, yielding successful... 

To investigate the validity of this hypothesis, we looked at the series of complexes, cis-Os(CO)4PW. Several of its members were reported, and even an apparent hydridomethyl complex was observed spectroscopically in small quantities (10,11). Furthermore, there was reason to believe that Os(CO)4 would be a very high energy fragment and hence that mechanisms other than simple... 

Hunt, J.E., MacFarlane, R.D., Katz, J.J., and Dougherty, R.C. 1981. High-energy fragmentation of chlorophyll a and its fully deuterated analog by 252Cf plasma desorption mass spectrometry. J. Am. Chem. Soc. 103 6775-6778. 

The laser ablation technique was first used to synthesize C-Ns by Niu et al. [12] wherein high-energy fragments react with nitrogen atoms generated independently by an RF-plasma discharge. This technique attained a... 

---