Hexose diphosphatase

The phosphoenolpyruvate may then be converted to fructose diphosphate by reversal of steps 10, 9,8, 7 and 5 in the glycolytic sequence shown in Fig. 9.4.This is then converted to fructose-6-phosphate by hexose diphosphatase and then to glucose-6-phosphate by the reverse of step 2 and finally to glucose by glucose-6-phosphatase.The glucose may then eventually be used to provide energy. The energy balance sheet may be prepared as follows ... 

Racker 56) demonstrated the synthesis of carbohydrates from carbon dioxide and hydrogen in a cell-free system by bringing together many of the enzymes listed in Table IV. A spinach fraction furnished the phospho-pentokinase, carboxydismutase, phosphopentosisomerase, transketolase, transaldolase, and hexose diphosphatase. To this fraction were added the other enzymes, DPN+, ATP, and a hydrogenase preparation. The hydro-genase enzyme furnished DPNH in the presence of hydrogen. When this mixture was incubated at 25° for 60 minutes, the synthesis of fructose 6-phosphate could be demonstrated. 

Lactic, pyruvic and 2-oxoglutaric acids Hexose diphosphatase (EC 3.1.3.11) 15.5... 

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