1,2,3,4,5,6- Hexachlorocyclohexane techniques

Lastly, we will describe how these methods have been used to characterize the effects of hexachlorocyclohexanes on neutrophil functions. Although we are limiting this to a discussion of neutrophils, these approaches are completely generalizable to other cell types and have already been applied to some. We hope this summary will be useful to workers who wish to extend these techniques to the cell systems of interest to them. 

Co(TPP) has been demonstrated to act as a catalyst for the electrocarboxylation of benzyl chloride and butyl bromide with CO - to give PhCHiCfOiOCH Ph and Bu0C(0)C(0)0Bu, respectively. The propo.sed mechanism involved Co(TPP)R and [Co(TPP-N-R) as intermediates (the latter detected by spectroscopy) in the catalytic production of free R or R-, which then reacted directly with Co(TPP) precipitated on graphite foil has been successfully used for the determination of organic halides, including DDT and 1,2,3,4,5,6-hexachlorocyclohexane (lindane), to sub-ppm level in aqueous solution. Deoxygenation of the solutions is not required, and the technique is moderately insensitive to the ionic composition of the solution. ... 

There is a potentially dangerous reaction of carbon tetrachloride with dimethylformamide in presence of iron. The same occurs with 1,2,3,4,5,6-hexachlorocyclohexane, but not with dichloromethane or 1,2-dichloroethane under the same conditions [1], A quantitative study of the reaction by DSC and ARC techniques shows that in a 1 1 wt. mixture with carbon tetrachloride in absence of iron, an exothermic reaction sets in below 100°C. Under adiabatic conditions, the heat release (207.6 J/g) would take a runaway reaction to over 240°C. In presence of 3% of iron powder, the same mixture shows 2 exotherms, one at 56°C (108 J/g) and the second at 94°C (275 J/g), a final adiabatic temperature exceeding 285°C being possible [2], Dimethylacetamide behaves similarly but more so. 

Boehncke, A., K. Martin, M. G. Muller, and H. K. Cammenga, The vapor pressure of lindane (g-1,2,3,4,5,6-hexachlorocyclohexane) - A comparison of Knudsen Effusion Measurements with data from other techniques , J. Chem. Eng. Data, 41, 543-545 (1996). 

Moreover, irrespective of degradation technique chromatography has been used as an effective monitoring tool in the biodegradation of hexachlorocyclohexane (HCH). The potential biodegradation of HCH isomers was evaluated using gas chromatography-mass spectrometry (GC-MS). " ... 

---