Heteroduplex formation

Volkov, A.A., Shao, Z. and Arnold, F.H. (1999) Recombination and chimeragenesis by in vitro heteroduplex formation and in vivo repair. Nucleic Acids Research, 27, el8. 

Fig. 4.1. Schematic representation of heteroduplex formation and principle of DGGE. Mutant homoduplexes (M) melt at a lower denaturant concentration than non-mutant homoduplexes (N) and are consequently retarded in the gel. Heteroduplexes (N + M) melt at even lower denaturant concentrations (modified from Borresen, 1996).

Table 8.2.6 Typical conditions for PCR amplification and heteroduplex formation...

The Study of Nucleic Acid Folding and Heteroduplex Formation. . 54... 

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