Heterodimerization rapamycin

Schlatter S, Senn C, Fussenegger M. Modulation of translation-initiation in CHO-Kl cells by rapamycin-induced heterodimerization of engineered eIF4G fusion proteins. Biotechnol. Bioeng. 2003 83 210-225. 

Although early heterodimerization studies used molecules such as FK-CsA, the most common approach is the use of rapamycin, which naturally functions... 

Another system uses C20-methallyl rapamycin (Ma-rap Fig. 4.2-5), which is unable to bind wild-type FRB and is therefore devoid of FRAP/mTOR inhibitory activity [22]. Ma-rap was found in a screen to bind very specifically to a triple mutant of FRB known as PLF [22]. Using the PLF mutant of FRB, Ma-rap can be used to achieve highly selective heterodimerization of proteins... 

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