Herpes simplex virus ribonucleotide reductase

Massie, B., Dionne, J., Lamarche, N. et al. (1995) Improved adenovirus vector provides herpes simplex virus ribonucleotide reductase R1 and R2 subunits very efficiently. Bio/Technology (Nature Publishing Company), 13 (6), 602-608. 

Mann, G. J., Gr%oslund, A., Ochiai, E., Ingemarson, R., and Thelander, L., 1991, Purification and characterization of recombinant mouse and herpes simplex virus ribonucleotide reductase R2 subunit. Biochemistry 30 1939nl947. 

EPR study of the mixed-valent diiron sites in mouse and herpes simplex virus ribonucleotide reductases. Effect of the tyrosyl radical on structure and reactivity of the diferric center. Biochemistry 36, 9093-9100. 

Atta, M., Andersson, K. K., Ingemarson, R., Thelander, L., and Gr%oslund, A., 1994, EPR studies of mixed-valent [Fe Fe ] clusters formed in the R2 subunit of ribonucleotide reductase from mouse or herpes simplex virus Mild chemical reduction of the diferric centers. J. Am. Chem. Soc. 116 6429n6430. 

When a virus infects a cell, it could induce its host to make deoxyribonucleotides for viral DNA replication by means of the cellular enzyme (probably derepressed after infection), or the virus could carry its own specific ribonucleotide reductase genes which are expressed in the host cell and produce a new enzyme. Ribonucleotide reductase of herpes simplex virus is even considered the transforming function of However virus-... 

Thus, the antiviral activity of the thiosemicarbazones has been correlated, in one study, with their ability to inhibit ribonucleotide reductase [8]. Methisazone inhibits the RNA-dependent DNA polymerase of Rous sarcoma virus (RSV) and inactivates its ability to transform chick-embryo cells [9], as well as inactivating herpes simplex [10] and some RNA viruses [11]. The activity against RSV is Cu-dependent [9], the Cu complex also being capable of inhibition of the polymerase [12]. Since these enzymes are zinc-dependent, interference with the role of zinc is possible. The inhibition of influenza virus-associated RNA transcriptase by... 

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