Herbal Supplements with Digoxin-like Immunoreactivity

Emit of detection was 4 ng mL and the limit of quantitation was 10 ng mL Bauer et al. described HPLC combined with UV detection for the determination of hyperforin and HPLC combined with fluori metric detection for the determination of hypericin and psudohypericin in human plasma. They used liquid-liquid extraction. The limit of quantitation was 10 ng mL for hyperforin and 0.25 ng mL for both hypericin and pseudohypericin. 

6 ngmL. 2 Riedel et al. also described an HPLC method combined with MS-MS for the determination of hypericin and hyperforin concentrations in human plasma. They used Equid-liquid extraction with ethyl acetate and hexane and used a reversed-phase (RP-18) column for analysis. The Emit of quantitaion was 0.05 ng mL for hypericin and 0.035 ng mL for hyperforin. The hypericin assay was linear between 0.05 and lOngmL and the hyperforin assay between 0.035 and lOOngmL 

3 HERBAL SUPPLEMENTS WITH DIGOXIN-LIKE IMMUNOREACTIVITY 

HPLC OF ACTIVE INGREDIENTS IN HERBAL NUTRITIONAL SUPPLEMENTS 

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