Heparin derivatives, activated partial

Hog mucosal heparin and its partially Af-desulphated derivatives retained biological (anticoagulant) activity after reaction with 5-isothiocyanatofluorescein to yield the corresponding A -fluoresceinylcarbamoylheparins. ... 

Heparanase and type IV collagenase activities were assessed by a method described previously [17]. Briefly, H-labeled heparin sulfate (HS) was incubated at 37°C with partially purified heparanase in the presence or absence of chitin derivatives. [ H]HS degradation products in the supernatant were analyzed by size-exclusion chromatography. Heparanase activity was determined by measuring the radioactivity of each fraction. H-labeled type IV collagen was incubated at 37°C for 48 h in the presence or absence of chitin derivatives. Type IV collagenolytic activity was calculated from the radioactivity in the supernatant. 

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