Heparin chromatographic methods

Chromatographic methods (essentially using ion-exchange resins) are especially useful for separating heparin from other glycosaminoglycans, for further analytical and structural characterization. However, it should be realized that recovery of heparin from resins is seldom quantitative,42 probably because of irreversible absorption of heparin fractions in the... 

Liquid chromatographic methods for routine heparin analysis have since been perfected. See S. Beni, J. F. K. Limtiaco, and... 

ES-HS has been isolated from medium of endothelial cell cultures via several chromatographic methods as described earlier. R ioselective modified heparin derivatives were prepared as described earlier . ... 

For detailed description and discussion of methods of separation and characterization of GAG, the reader is referred to specific mono-graphs38-42-46-47 dealing with the advantages and drawbacks of different colorimetric, titrimetric, electrophoretic, chromatographic, spectroscopic, and enzymic methods for the qualitative and quantitative characterization of heparin and its most common contaminants. The present article is concerned only with analytical aspects of relevance to the structural characterization of heparin. 

In addition to ascorbic acid, uric acid is an important antioxidant in blood plasma (9). To assess the antioxidative capacity of plasma it is useful to determine ascorbic acid and uric acid in a single chromatographic run. Ascorbic acid and uric acid have been measured simultaneously from heparinized plasma by UV detection (64). To optimize the detection during method setup, the sample was dissolved in the mobile phase, pH 5.5, where ascorbic acid had the absorbance maximum at 262 nm and uric acid at 285 nm. The wavelength of 262 nm was chosen because ascorbic acid is present at a lower concentration than uric acid in plasma. The same mobile-phase pH (5.5) and UV detection at 280 nm for... 

Verhaeghe, T. Diels, L. De Vries, R. De Meulder, M. de Jong, J. Development and validation of a liquid chromatographic-tandem mass spectrometric method for the determination of galantamine in human heparinized plasma, J.Chromatogr.B, 2003, 789, 337-346. 

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