Hen s egg chorioallantoic

Luepke, N.P. (1985). Hen s egg chorioallantoic membrane test for irritation potential. Food. 

Several in vitro methods, including the hen s egg chorioallantoic membrane test (HET-CAM) the bovine cornea opacity and permeability assay (BCOP) and the isolated rabbit eye (IRE) test, have gained regulatory acceptance in Europe for the classification of severe eye irritants. Many companies are using such techniques successfully to reduce in vivo testing during development. ... 

ICCVAM (2009). Background review document—current status of in vitro test methods for identifying mild/moderate ocular irritants Hen s egg test—chorioallantoic membrane test method. National Institute of Environmental Health Sciences, Resesarch Triangle Park, North Carolina, USA. Available at http //iccvam.niehs.nih.gov/ docs/ocutox docs/InVitro-2010/AppE-HET-Front-Body.pdf. Accessed 31 July 2013... 

The test used to prove the safety of Centipeda s extract the Hen s Egg Test utilizing the chorioallantoic Membrane (HET-CAM) which proved to be more sensitive to liquid irritants than is the rabbit eye (and obviously human skin). The extract at 10% concentration had practically no ocular irritation potential. 

The hen s egg test on the chorioallantoic membrane (HET-CAM assay) enables irritant reactions to be identified that are similar to those which occur in the eye using the standard Draize rabbit eye test. In the HET-CAM test system, three reactions are determined, namely, haemorrhage, lysis and coagulation (sometimes hyperemia is also used as a parameter) of the CAM on the ninth day of embryonation when nerve tissue and pain perception have not yet developed (Figure 9.1.4). 

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