Hemostasis, Thrombosis, and Coagulation

It is not known whether blood coagulation takes place continuously in vivo (Hjort and Hasselback, 1961 Spaet, 1963). The observation that coagulation proteins have a short half-life is frequently cited as evidence in favor of the concept. Although platelets and fibrin have been fotmd on abnormal endothelium, it bas never been conclusively shown that they can be deposited on normal endothelial tissue, although Mustard et al. (1964b) believe that platelet thrombi can occur on apparently normal endothelium. The question is far from settled, but is of considerable importance since fibrin or platelet masses may form a nidus for atherosclerotic plaques (Duguid, 1954 Mustard et al., 1964b). For further details see O Brien (1964) and Marcus and Zucker (1965). 

Another useful system is the thromboplastin generation test (TGT) (see Biggs and Macfarlane, 1962, for detailed description). In this 

Platelets recovered from above plasma and washed  

There are many other coagulation tests described in the literature which investigators have used to evaluate the lipid materials. These include the whole blood clotting time in glass or silicone-treated tubes, the thrombin generation test, and the prothrombin consumption test using clotted blood or platelet-free native plasma. In general they are modifications or variations of the basic principles involved in the assays mentioned above. 

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