Hemoglobin solubility

The acute hemolysis test The results of the hemolysis test are presented in table 4. The absorbance is based on the hemoglobin solubility from the erythrocytes. Thus, the absorbance corresponds to the number of hemolyzed erythrocytes. The data directly reflected if newly prepared porous AI2O3... 

One Anson unit is the amount of enzyme that, under standard conditions, digests hemoglobin at an initial rate, Hberating per minute an amount of TCA-soluble product which produces the same color with phenol reagent as one milliequivalent of tyrosine (91). 

Heme (C34H3204N4Fe) represents an iron-porphyrin complex that has a protoporphyrin nucleus. Many important proteins contain heme as a prosthetic group. Hemoglobin is the quantitatively most important hemoprotein. Others are cytochromes (present in the mitochondria and the endoplasmic reticulum), catalase and peroxidase (that react with hydrogen peroxide), soluble guanylyl cyclase (that converts guanosine triphosphate, GTP, to the signaling molecule 3, 5 -cyclic GMP) and NO synthases. 

Most nitrites are soluble in water and mildly toxic. Despite their toxicity, nitrites are used in the processing of meat products because they retard bacterial growth and form a pink complex with hemoglobin that inhibits the oxidation of blood (a reaction that would otherwise turn the meat brown). Nitrites are responsible for the pink color of ham, sausages, and other cured meat. 

Itano, H. A. "Solubilities of Naturally Occurring Mixtures of Human Hemoglobin". Arch. Blochem. and Blophys., (1953),... 

Weickert MJ, Pagratis M,Glascock CB, et al. A mutation that improves soluble recombinant hemoglobin accumulation in Escherichia coli in heme excess. Appl Environ Microbiol 1999 65 640. 

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