Heart transaminase activities

Apart from hormonal aspects of pseudocholinesterase activity previously dealt with (48,86,107), there have been a number of studies since 1954 showing that cortisone may act on the transaminase activity of various organs (46, 49, 103), particularly liver, heart, and kidneys, which have the highest activity (4,21). In 1957 Gavosto et al. (58) suggested that the hormone may enhance gluconeogenesis by activating the transamination process in the liver. Rosen et al. (104-106) found... 

In this experiment, you will perform a three-step purification of glutamate-oxaloacetate transaminase from pig heart. With each step in the purification, you will assay different fractions for GOT activity and total protein concentration. These values will then be used to calculate the total activity, specific activity, and percent yield of GOT following each purification step. The goal of the experiment is to achieve 80- to 100-fold purification of the enzyme with >5% yield. The overall effectiveness of the purification procedure will be analyzed by SDS-PAGE on the last day of the experiment. Be sure that you determine three different... 

Transamination reactions combine reversible amina-tion and deamination, and they mediate redistribution of amino groups among amino acids. Transaminases (aminotransferases) are widely distributed in human tissues and are particularly active in heart muscle, liver, skeletal muscle, and kidney. The general reaction of transamination is... 

Perfused heart incorporates at high level into C-2, C-3, and C-4 of glutamate due to TCA cycle activity and activity of glutamate-oxalaocetate transaminase. No label is incorporated into alanine (pyruvate). Some incorporation into aspartate if glucose is in the perfusion medium. 

Determination of ALT ALT (formerly glutamate pyruvate transaminase) catalyzes the equilibrium transfer reaction of the amino group from L-alanine to 2-oxoglutarate to form L-glutamate and pyruvate ALT requires pyridoxal phosphate as coenzyme, which acts as an amino carrier. It is found in the main organs, such as the liver, kidney, and heart. The ALT activity in serum is elevated in diseases of the liver. 

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