Harvest and Pre-fermentation Processing

Piocosiing The mots are harvested when they reach markatable size. There Is little or no processing except In the Orrent, where they may be made into fermented and pickled pre parations. 

A. vinelandii UWD and W. eutropha were grown in a two-step process, employing a pre-culture and a polymer medium. For PEG and DEG modulated fermentation, A. vinelandii UWD was used. After 48 h of growth, 10 vol.% of the pre-culture were used as inoculum for the polymer medium, which was harvested after 24 h (12). Both media were amended with 2.0 wt % of the respective PEGs. It is noteworthy that A. vinelandii UWD must be grown in the presence of 20 mg/L rifampicin, in order to prevent the back mutation of the engineered strain to the A. vinelandii UW parental strain genotype, which is not resistant to the antibiotic. All fermentation experiments were conducted in a shaker-incubator (New Brunswick Scientific) at 30 °C and 180 rpm. Cells were... 

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