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Haptens target, biotinylation

Sandwich-Type Assay Based on Biotinylation of a Target Hapten. 145... [Pg.139]

Fig. 6. Principle of a noncompetitive hapten immunoassay based on quantitative biotinylation of the target molecule (A) biotinylation of a hapten with reactive amino group(s) and subsequent (B) sandwich-type immunoassay procedure. Fig. 6. Principle of a noncompetitive hapten immunoassay based on quantitative biotinylation of the target molecule (A) biotinylation of a hapten with reactive amino group(s) and subsequent (B) sandwich-type immunoassay procedure.
Similarly, Coutlee et al. (1989a) developed a sandwich assay in which (i) hybrids formed in solution between the biotinylated hapten probe and the target were collected by anti-biotin antibodies immobilized in wells of a microtiter plate and (ii) detected by /3-galactosi-dase-Fab fragment (of monoclonal antibody to RNA DNA) by conversion of a substrate to a fluorescent product. Optimum conditions included hybridization for 16 h at 75°C in 2 X SSC, 10 mM HEPES, using 0.1 xg/ml probe with 7% biotinylation. [Pg.176]

See other pages where Haptens target, biotinylation is mentioned: [Pg.145]    [Pg.377]    [Pg.144]    [Pg.399]    [Pg.176]    [Pg.157]   
See also in sourсe #XX -- [ Pg.145 , Pg.146 , Pg.147 , Pg.148 ]



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