HALL Glass containers

Strip lighting in a classroom, hospital, business hall or kitchen is often called fluorescent lighting, although in fact it is a phosphorescent process, as above. Each bulb consists of a thin, hollow glass tube that is sealed at both ends. It contains gas such as helium, argon or krypton, and a drop of liquid mercury (about 0.5 mg of mercury per kilogram of lamp, or 0.5 parts per million). Like the neon and sodium lamps above, the pressure inside the tube is about 30 Pa, so the mercury evaporates to become a vapour. It is the mercury that yields the light, albeit indirectly. 

Kolthoff H. A. Laitinon, pH and Electrotitrations, J. Wiley, NY (1941) 10) M. Dole, The Glass Electrode, J. Wiley, NY (1941) (Contains complete bibliography) 11) H.T.S. Britton, Hydrogen Ions, Chapman Hall, London (1942) 12) J. Reilly W N. Rae, Physico-chemical Methods, Van Nostrand... 

Once washed, type C chloroplasts (Hall, 1972) were prepared by conventional procedures from young pea leaves (Pisum sativum var Feltham first). Chloro-plast ATPase was measured in a thermostatted glass vessel fitted with a pH-sensitive electrode. The medium contained 50 mM KCl, 2 mM tricine-NaOH, pH 8.0, 5 mM MgCl2, 0.1 mM EDTA, 5 M pyocyanine and 1 mM KP. and 10-20 g chlorc phyll/ml. For DTT activations the medium also contained 4 mM DTT and was illuminated for 4 minutes, while for trypsin activation, illumination was with 1 Kig/ml trypsin for 30 seconds, followed by the addition of an excess of trypsin inhibitor. Light was provided by a 150 W projector lamp. After the indicated periods of darkness (see each figure), MgATP was add d to a final concentration of 2 mM and the resulting ATPase activity monitored by fall in pH. Basal activity was that without prior illumination. 

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