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Guanylate cyclase assay

Pietta PG, Mauri PL, Gardana C, Benazzi L. Assay of soluble guanylate cyclase activity by isocratic high-performance liquid chromatography. J Chromatogr B Biomed Sci Appl 1997 690 343-347. [Pg.237]

DEVELOPMENT OF A NOVEL BIOLUMINESCENT ASSAY FOR NITRIC OXIDE BY USING SOLUBLE GUANYLATE CYCLASE... [Pg.109]

ANP is a polypeptide hormone that is secreted in large quantities by the atrial portion of the heart and from a variety of other mammalian cell types. It exerts many of its actions via activation of particulate guanylate cyclase [16, 134]. ANP is present in human follicular fluids [163] and specific ANP receptors have been identified in human spermatozoa [144]. Sperm chemotaxis to ANP was demonstrated by sperm accumulation in capillaries with ascending [6] and descending [192] gradients and by choice assays [192] (Table 4). It is not yet known whether ANP is involved in sperm chemotaxis in vivo and whether the physiological chemoattractant for human spermatozoa is an ANP-like substance. Since chemotaxis to ANP at physiological concentrations can be observed only in the presence of a neutral endopeptidase inhibitor such... [Pg.433]

In conclusion, follicular fluid, bourgeonal, ANP, and RANTES are the only substances that have been demonstrated to act as chemoattractants for spermatozoa by assays that differentiate between chemotaxis and other processes causing accumulation (Table 1). ANP may not be a direct physiological chemoattractant but rather a guanylate cyclase activator. The significance of sperm chemotaxis to bourgeonal and RANTES is an open question. The identity of the chemoattractant(s) in follicular fluid has yet to be revealed. [Pg.436]


See other pages where Guanylate cyclase assay is mentioned: [Pg.34]    [Pg.376]    [Pg.45]    [Pg.308]    [Pg.109]    [Pg.110]    [Pg.298]    [Pg.413]    [Pg.16]   
See also in sourсe #XX -- [ Pg.308 , Pg.310 ]




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