Guanidination of Amines

Protocols for guanidination reactions typically use basic conditions to deprotonate all the lysine s-amino groups, which is necessary to achieve efficient yields. The amount of N-terminal 

Prepare 5 pil of a peptide solution to undergo guanidination at a concentration of 1 pmol/pil and add 5.5 pi of 7 N ammonium hydroxide. 

5 pi of the o-methylisourea hemisulfate solution to the peptide solution with mixing. 

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