Ground-glass cell

Histologically, the HBsAg-containing hepatocytes have the appearance of so-called ground glass cells (S. Hadzi-YANNisetal., 1973). (163) (s. figs. 5.7 22.8) (s. pp 114, 396)... 

Fig. 22.8 Immunohistochemical detection of HBeAg in the nuclei of liver cells shown by monoclonal antibody. Hepatocytes with ground glass-like homogenization of the cytoplasm, so-called ground glass cells (arrow). HBsAg in the cytoplasm is not presented immunohistochemically in this case (s. figs. 5.7 22.7)...

Diffusion Cell System A diffusion cell system with a standard open cap ground glass surface with 15 mm diameter orifice and total diameter of 25 mm. 

The Tiselius cell consists of a U-tube of rectangular cross-section, which is divided into a number of sections built up on ground-glass plates so that they can be moved sideways relative to one another. The protein solution is dialysed against buffer (to avoid subsequent disturbance of the boundary by osmotic flow), and then the sections of the cell are filled with buffered protein solution or buffer solution, for example, as shown in Figure 7.8. Large electrode vessels containing reversible electrodes are then attached, and the whole... 

The large variety of commercially available ground-glass joints, glass frits, and stopcocks, and the services of a glassblower make it easy to fabricate cells for special purposes, and a selected few of these are described in a later section. Most companies that sell electrochemical instrumentation offer a number of cells of different styles, as well as electrodes and other accessories. 

Figure 6.20 Flow cell with horizontal DME for continuous monitoring (a) sample introduction tube (b) solution outlet (c) DME capillary (d) O-ring seals (e) waste mercury (/) agar salt bridge to counter electrode (g) SCE reference electrode with agar plug and fine glass frit (h) ground-glass joints.

Fig. 1. Kubitschek style chemostat. A, Air pump B, hosecock clamps (closed during autoclaving) C, growing culture D, air exit port E, ground glass joints F, syringe filter, G, gang valve H, bottle containing water I, input area K, heating tape M, fresh medium O, siphon P, peristaltic pump R, rubber stopper with holes S, splash shield T, silicone rubber tubing W, waste Y, waste collection tube. Circles in the liquids represent bubbles. Stippling indicates media with cells.

The three kinds of open cells, i.e., the open-cup, the draft and the touch-flow cell, are, however, the same to the effect that each has the ground-glass joint at the upper end. A difference in the three is only that the thin gas-permeable silica fiber sheet, which is used to support a reference material, or a gas-permeable oxidatively-heating substance, tested on the bottom of the draft cell, is not used in both the open-cup cell and the touch-flow cell. ... 

Figure 75. This photograph shows a general view of the referenee cell assembly including the glass vent tube eombined with the empty open-cup cell. The assembly is held in the venical position by means of a clamp of a stand. It is possible to slide the stand horizontally on the laboratory desk. On the other hand, a laboratory jack, at the center of the upper plate of which a pedestal is stuck, is fixed on the desk. The position of the stand is then adjusted horizontally in order that the assembly may be aligned vertically with the pedestal. A pack of cigarettes is put on the laboratory jack, and by the side of the assembly, to show the relative size of the open-cup cell. The dimensions of the open-cup cell arc almost the same as those of the closed cell, i.e., 1.4 cm in outside diameter, about 3 cm in length, except the ground-glass joint at the upper end.

Figure 76. This is an enlarged view of the reference cell assembly including the empty open-cup cell and the pedestal. The cell is seen to be supported with a thin aluminium washer and two springs and is combined with the ground glass joint at the lower end of the glass vent tube.

Figure 79. The empty open-cup cell is separated from the ground glass joint of the glass vent tube by lowering the laboratory Jack.

Figure 81. The reference cell thus prepared is then combined concentrically and softly with the ground glass joint of the glass vent tube by raising again the laboratoiy jack to constiuet the reference cell assembly in the meantime, the CA thermocouple, covered with a glass capillary tube, to measure the Tr,f, the temperature of an oxidatively-heating substance, or that of a non-volatile chemical of the TD type, is inserted automatically and axially to the longitudinal center of silica of coarse particles charged in the cell.

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