Green fluorescent protein Subject

In the years immediately following, this procedure was optimized to express real proteins, the first being - for obvious detection reasons - the green fluorescence protein (GFP). Protein expression is actually the subject of the next section. 

With the advent of very high resolution fluorescence microscopes it is perhaps not surprising that new approaches have been developed to study the movement of materials through cells. In particular, the gene for the naturally fluorescent green fluorescent protein (GFP) of the jellyfish Aequoria victoria has been cloned, isolated and subjected to deliberate genetic modification. GFP has been... 

Figure 4. Fluorescence microscopy image on Oregon Green labeled streptavidin subjected to the SMAP-treated, 5x5 gw Ti02 in S102 substrate. Streptavidin adsorption can only be observed on the Ti02/DDP spots, while the Si02/PLL-g-PEG remains protein resistant. The inset shows the local distribution of fluorescence of the Oregon Green labeled streptavidin across the surface (in arbitrary units). A contrast of 100 1 was observed.

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