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Gradient programming, high-performance

One standard method approach that appears to have wide application in the pharmaceutical industry is the use of reversed-phase high performance liquid chromatography (HPLC) with a wide solvent gradient program. For example, a linear gradient from 95%... [Pg.43]

FIG. 8 High-performance liquid chromatogram of the pigments extracted from pumpkin seed oil. Elution program solvent A 100% methanol solvent B methanol-hexane (4/1 v/v). Linear gradient solvent B from 0% to 100% in 28 minutes and then isocratic elution for 6 minutes. [Pg.64]

Ricci, M.C. Cross, R.F. High performance liquid chromatographic euialyses of sulphonamides and dihydrofolate reductase inhibitors. II. Separations in acetonitrile modified solutions, ternary gradient studies flow programming. J.Liq.Chromatogr. Rel.Technol., 1996,19, 547—564... [Pg.1274]

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Gradient program

Gradient programming