Golgi apparatus, ARFs

Arf Vesicular transport between endoplasmatic reticulum and cis-Golgi apparatus... 

Golgi apparatus, the nucleus, and the plasma membrane (Donaldson and Klausner, 1994). The identification of ARFs as activators of an isoform of phospholipase D (Brown etal., 1993 Cockcroft eta/., 1994 /Vlassenburg ef a/., 1994 Hammond et a/., 1995), which appears to be present (at least in part) in the Golgi apparatus (Ktistakis etal., 1995), has helped to define further the function of ARF in cells. Investigations into the role of ARFs in these processes have employed recombinant wild-type and mutant ARF constructs. Thus, the assays discussed here have become commonly used to measure native ARFs, and to assess the integrity of recombinant proteins and the effects of mutations on their activity. 

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