Glycosidic linkages correction factors

Because the galacturonic acid units of pectin spend a significant portion of the time in the polymerized form, there is less decomposition than that of galacturonic acid standards subjected to the same conditions of hydrolysis. For this reason, correction factors for hydrolysis losses, under any conditions, can never be completely accurate therefore, it is preferable to cleave glycosidic linkages with the minimum of decomposition. 

The rationale for the correct setting of current knowledge about the shape of polysaccharides in solution is based on three factors the correlation between primary structure (i.e., the chemical identity of the carbohydrates polymerized in the chain), intrinsic conformational features dictated by the rotational equilibria (often the major contributions are due to the rotation about the glycosidic linkages) and the interaction with the other mo-... 

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