Cluster glycoside effect

FIGURE 16.5 A schematic representation of (a) SPR competitive and (b) direct binding assays of Con A-Man recognition, (c) The structure of clicked [G3]-Man glycodendrimer used for studying the glycoside cluster effect. 

Glycoside cluster effect A mnltivalency effect, based on the interaction a particle exposing saccharide ligands and a mnltimeric protein. 

Triton-solubilized, affintiy-purified HL still expresses glycoside cluster effect, albeit to a much lesser extent than the HL on the hepatocyte surface. The best di-valent oligosaccharide, PENTA-2,4, had 20-fold higher affinity than the mono-antennary structure, as compared to 1000-fold for the hepatocyte surface receptors, while the best triantennary oligosaccharide, NONA I, had 700-fold higher affinity over the monovalent structure, as compared to 100,000-fold enhacement for the hepatocyte system. Presumably organization of subunits in the soluble form is somewhat relaxed compared to the cell surface HLs. 

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