Glycoproteins gold labeled

Protein-labeled colloidal gold probes suit as well as for immuno-histochemistry in electron microscopy as for detection of antigens or glycoproteins on blots. There are several protocols for enhancement of electron density and visible color resulting in higher sensitivity. 

Horisberger, M., Rosset, J. and Vonlanthen, M. 1977. Location of glycoproteins on milk fat globule membrane by scanning and transmission electron microscopy, using lectin-labelled gold granules. Exp. Cell Res. 109, 361-369. 

Lectins, or proteins with specific binding sites for carbohydrates, can be used as targeting molecules to localize particular glycoconjugates such as glycoproteins or glycolipids on cell surfaces (Fig. 373). Labeled with gold particles, lectins are important probes for detection of cell surface components and intracellular receptors and in immunological or biochemical assay procedures (Bog-Hansen et al., 1978 Kimura et al., 1979 Nicolson, 1978 Roth, 1983 Benhamou et al., 1988 Nakajima et al., 1988). 

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