Glycoproteins fluorescein-5-thiosemicarbazide

To the purified, oxidized glycoprotein(s), add fluorescein-5-thiosemicarbazide to a final concentration of 0.5 mg/ml. 

The methods for using this reagent in labeling glycoproteins on cell surfaces or in solution are similar to those described for fluorescein-5-thiosemicarbazide, above. 

Generalized protocols for the use of hydrazine-probes reactive toward aldehyde residues can be found in Section 1.1. These procedures are directed at the labeling of cell surface glycoproteins or glycoproteins in solution. Substitution of Lissamine rhodamine B sulfonyl hydrazine for the fluorescein-5 -thiosemicarbazide reagent described in that section can be done without difficulty. Some optimization may be... 

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