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Glycoprotein Syntheses in Glycomics

The hydroxyl groups of the carbohydrates are protected as acetyl or benzoyl ester which, upon cleavage of the completed glycopeptide from the solid support, are readily removed by treatment with sodium methoxide or hydrazine. [Pg.675]

The technology common to DNA and proteins has become realized in the form of biochips, which include carbohydrate arrays or glycochips. The emergence of biochips has revolu- [Pg.675]

X = leaving group Pi Psi Pe=Orttiogonally removal protecting groups [Pg.676]

General procedures to manufacture, application and management of microarrays have been described in section 14.3. Special concerns to prepare carbohydrate micioar-rays will be dealt with in this subsection. Two methods are used to immobilize glycans on the surface for the fabrication of glycochips (Feizi et al., 2003) noncovalent and covalent immobilization technologies. [Pg.676]

1 Non-covalent immobilization. Unmodified glycans of high molecular weight (Mr 20kDa) can be inunobilized nonspecifically on nitrocellulose-coated [Pg.676]


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