Glycoproteins high mannose type

Peripheral to the Gal-GlcNAc sequences of glycoproteins, there can occur an array of blood-group-related glycosylations, anionic substitutions (sialy-lation, 0-sulfation, and 0-phosphorylation), or both. Chains of the high-mannose type may also bear phosphate groups. Table VI reviews the pat-... 

A saitoi acid carboxypeptidase is a glycoprotein that contains both N- and 0-1 inked sugar chains [81, 82], Glycosylation of the A. saitoi carboxypeptidase suggests the stability of this enzyme against proteinase and proteolysis. In this chapter, the characterization of a new N-linked high-mannose type oligosaccharide from A. saitoi acid carboxypeptidase is described. 

Lace et aL proved that testicular hyaluionidase is a glycoprotein of the high-mannose type [70], They removed the carbohydrate moiety of die enzyme using endoglycasidase F and showed that this did not alter the activity and the antigenicity of the enzyme. 

Complex carbohydrates released from glycoproteins were readily profiled by capillary gel electrophoresis with LIF-based detection of 1-aminopyrene-3,6,8-trisulfonic acid (APTS)-labeled sugar molecules (Figure 10) [125]. High-mannose-type oligosaccharides of ribonuclease B were derivatized by APTS and separated by capillary electrophoresis using polyethylene oxide separation medium [126]. 

Gross V, Steube K, Tran-Thi TA, McDowell W, Schwarz RT, Decker K, Gerok W, Heinrich PC. Secretion of high-mannose-type a 1-proteinase inhibitor and a 1-acid glycoprotein by primary cultures of rat hepatocytes in the presence of the mannosidase 1 inhibitor 1-deoxymannojirimycin. Eur. J. Biochem. 1985 150(1) 41 6. 

T. Ogawa and T. Nukada, Synthesis of a branched mannohexaose, a part structure of a high-mannose-type glycan of a glycoprotein, Carbohydr. Res., 136 (1985) 135-152. 

Fig. 15. High mannose type triantennary glycan from glycoprotein Ilia.

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