Glycolipid immobilization

Figure 2 Immobilization strategies, (a) A schematic representation of noncovalent adsorption. The lipid tail of a neo-glycolipid adheres to a hydrophobic surface via noncovalent interactions, (b) Strategies used for covalent immobilization of glycans.

The GM3 in B16 cells is localized within glycolipid-enriched microdomains in the lipid membrane (37). The microdomains, often referred to as lipid rafts, also contain a variety of signaling proteins and enzymes. Binding of B16 cells to immobilized Gg3 activates the signaling proteins Ras and Rho, suggesting... 

Figure 3. AFM images of MBP-GalT immobilized on the glycolipid LB films. The density of glycolipid 1 in the matrix lipid 2 was (a) 0% (b) 5 %, (c) 10 %,...

Shah, V., Doncel, G. F., Seyoum, T. et al. (2005) Sophorolipids, microbial glycolipids with anti-human immunodeficiency virus and sperm-immobilizing activities. Antimicrob. AgetUs Chemother., 49, 4093-4100. 

Glycolipids can be incorporated in liposomes, directly captured on the matrix of the sensor chip (Figure 4), and completely removed after a measurement [22], In a similar approach using immobilized antibodies for liposome capture, bacterial toxin affinity and binding specificity for glycolipid receptors were determined [8]. 

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