Glycolipid epithelial cells

Glycolipids of Rat Small Intestine with Special Reference to Epithelial Cells in Relation to Differentiation... 

Compared with the B-active glycolipid found in non-epithelial tissue, the fucolipids in epithelial cells were based on glucosamine instead of galactosamine (see Fig. 9). The H active fucolipids of black and white rat had three and five sugars, respectively. The glycolipids found in epithelial cells of the two strains are summarized in Fig. 10. 

Fucolipids are present in both compartments. However, the blood group B active compound of non-epithelial cells (absent in epithelial cells) is based on GalNAc while the H and A active substances specific for epithelial cells have GlcNAc in their core saccharide. In fact, GlcNAc seems to be absent from all non-epithelial glycolipids. The minor fucolipid based on tetrahexosyl-ceramide (as indicated in fraction B of Fig. 5) was obtained from pooled tissue. This glycolipid has been shown to be located in the epithelial cells. 

Figure 11. Selected ion monitoring from mass spectrometry of glycolipids of epithelial cells of the two rat strains. A total of 200 fig each of the permethylated-reduced mixture was evaporated by a temperature rise of 5°C/min, and mass spectra were recorded each 38 sec. Electron energy was 34 eV, acceleration voltage 4 kV, trap current 500 fiA, and ion source temperature 290°C.

Figure 13. Protein and enzyme curves for epithelial cells fractionated from small intestine of the two rat strains. Experimental conditions were modified from Weiser (4). Pooled fractions used for glycolipid analysis are defined at the top. Data were from single rats.

In our initial studies reported here of glycolipids of rat small intestine, preparative and structural methods were adapted to characterize epithelial and non-epithelial tissue and epithelial cells of different location and level of differentiation. The two compartments were distinctly different with core saccharides with GalNAc being restricted to non-epithelial cells while those... 

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