Glycine isotopically labeled

Diagnostic enzyme assay that uses stable-isotope-labeled substrates to detect L-argi-nine glycine amidinotransferase deficiency. Clin Chem 49 803-805... 

Pyridines and pyrroles can be formed in different pathways by Mail-lard reaction. The formation of 5-methyl pyrrole aldehyde and 6-methyl-3-pyridinole has been observed by Nyhammar et al (17) by the reaction of isotope labelled 3-deoxyosone with glycine. The 3-deoxy-hexosone represents an -dicarbonyl compound and in this way the Strecker degradation occurs. Another pathway is the reaction of fu-rans with ammonia. Under roast conditions, we have obtained primarily the corresponding pyrrole, whereas we found the corresponding py-... 

With appropriate isotopic labelling experiments it was quickly demonstrated that this rearrangement involves the transposition of a glycine moiety between two adjacent carbon atoms rather than that of a carboxyl group. Furthermore, since no exchange of protons occurs between the substrate and the medium during the... 

The need for C-labeled analogues of DALA for the elucidation of biosynthetic and metabolic pathways towards heme and chlorophyll in living cells led to the development of other synthetic routes. The reaction of succinoyl chloride monoesters with C-labeled potassium cyanide provided a straightforward route towards acyl cyanides that are convenient precursors for DALA (24-27). More recently, a versatile and efficient syntheses of C-4-, C-5- and N-DALA was reported (28). The pathway for these syntheses was based on a chain elongation of isotopically labeled glycines. 

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