1-Glyceryl phosphate

Commercial kits are based on the Upase-catalyzed total hydrolysis of the triglyceride, followed by glycerol kinase-catalyzed synthesis of 1-glyceryl phosphate (67) and GPO-catalyzed oxidation of the latter, as shown in equation 17. The end analysis is by a chromogenic oxidation process catalyzed by peroxidase, similar to equation 27, involving 4-aminoantipyrine (81) and 3,5-dichloro-2-hydroxybenzenesulfonate (88) or iV-ethyl-iV-(3-sulfopropyl)-m-anisidine (94)28. 290... 

Glyceryl phosphate human serum determination, 630 hydrogen peroxide determination, 625, 626 triglyceride determination, 633 GOME (Global Ozone Monitoring Experiment), 605... 

Problem 29.13 Write a mechanism for step 6 of gluconeogenesis, the reduction of 3-phospho-glyceryl phosphate with NADH/H+ to yield glyceraldehyde 3-phosphate. 

Phosphatidic acid the structural backbone of the glycerophospholipids two molecules of fatty acids are esterified to a molecule of glyceryl phosphate. 

A determination of the pH dependence of the lanthanum hydroxide gel-promoted hydrolysis of /3-glyceryl phosphate revealed that the two maxima exist in the pH-rate profile, one at pH 8.6 which presumably involves the species La (OH)+2 and another (smaller) maximum at pH 10.4 which involves the species La (OH) 2+ (4). Presumably the same kind of catalytic mechanism is operative in both cases. These reactions may serve as models for the metal ion-promoted alkaline phosphatases which have been shown to proceed with P—O cleavage (and with no oxygen exchange). 

The repulsion between molecules having oily or aqueous properties is the basis for membrane construction. The lipids found in membranes are mostly based on glyceryl phosphate and normally contain three different side chains—one saturated, one unsaturated, and one very polar. 

The saturated chain is added first, at Cl of glyceryl phosphate. The reagent is a thiol ester called acyl coenzyme A, whose full structure you will see in the next chapter. This reaction occurs by simple nucleophilic attack on the carbonyl group of the thiol ester followed by loss of the better leaving group, the thiolate anion. Then the process is repeated at the second OH group where an unsaturated fatty acid, perhaps oleic acid, is added by the same mechanism. 

In a completely unrelated development, E. Bamann (7) showed that lanthanum hydroxide promotes the hydrolysis of glyceryl phosphate subsequently, BUTCHER and Westheimer (9) investigated the promotion of the hydrolysis of phosphate esters by lanthanum hydroxide gel, and found a more than thousandfold increase in the rate. Tliey also proposed a mechanism for the reaction which, however, involves water. 

Disodium a-glycerophosphate. See Disodium glyceryl phosphate Disodium glyceryl phosphate CAS 1555-56-2 EINECS/ELINCS 216-304-3 Synonyms Disodium a-glycerophosphate Classification Organic salt Empirical CsHeOeP 2Na Uses Emulsifier in cosmetics... 

Tri methyl phosphate C3H9O6P 2Na Disodium glyceryl phosphate C3H10N2... 

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